Adroher F J, Osuna A, Lupiáñez J A
Department of Parasitology, Faculty of Pharmacy, University of Granada, Granada, Spain.
Acta Trop. 1991 Nov;50(1):79-85. doi: 10.1016/0001-706x(91)90075-u.
We have investigated the effects of the metabolic inhibitors, phloretin, 2-deoxy-D-glucose, maleate and trans-aconitate, as well as two intermediates of the tricarboxylic-acid cycle, acetate and citrate, on the growth and metacyclogenesis of Trypanosoma cruzi. 0.1 mM phloretin increased the percentage of metacyclic forms about 2.7-fold without affecting growth rate, whereas the other inhibitors had no apparent effect on either growth or differentiation rates. The addition of 5 mM citrate stimulated differentiation by about 2.6-fold. When either 10 mM citrate or 10 mM acetate were added, on the other hand, both the growth and differentiation rates were severely inhibited.
我们研究了代谢抑制剂根皮素、2-脱氧-D-葡萄糖、马来酸盐和反乌头酸盐，以及三羧酸循环的两种中间产物乙酸盐和柠檬酸盐对克氏锥虫生长和循环体发育的影响。0.1 mM根皮素可使循环体形式的百分比增加约2.7倍，而不影响生长速率，而其他抑制剂对生长速率或分化速率均无明显影响。添加5 mM柠檬酸盐可使分化增加约2.6倍。另一方面，当添加10 mM柠檬酸盐或10 mM乙酸盐时，生长速率和分化速率均受到严重抑制。
