Osuna A, Adroher F J, Lupiáñez J A
Department of Parasitology, Faculty of Pharmacy, University of Granada, Spain.
Cell Differ Dev. 1990 May;30(2):89-95. doi: 10.1016/0922-3371(90)90077-a.
The influence of electrolytes and non-electrolytes, especially NaCl and sorbitol, on the metacyclogenesis and growth of Trypanosoma cruzi has been studied. The addition of 50 or 100 mEq/l NaCl to the culture media significantly increased the development of metacyclic forms. Other electrolytes and non-electrolytes had no effect on epimastigote-metacyclic differentiation. The growth rate was never modified to any extent. The influence of sodium concentration, osmotic pressure, among other factors, are discussed. Electrophoresis showed proteins bands which could be related either to the adaptation of T. cruzi to the new culture media or to the initiation of differentiation processes.
已经研究了电解质和非电解质，尤其是氯化钠和山梨醇，对克氏锥虫循环后期发育和生长的影响。向培养基中添加50或100 mEq/l的氯化钠可显著增加循环后期形式的发育。其他电解质和非电解质对前鞭毛体-循环后期分化没有影响。生长速率从未在任何程度上被改变。讨论了钠浓度、渗透压等因素的影响。电泳显示出蛋白质条带，这些条带可能与克氏锥虫对新培养基的适应性或分化过程的启动有关。
