通过化学发光法测量血液和血浆中的亚硝酸盐：陷阱与解决方法
The measurement of blood and plasma nitrite by chemiluminescence: pitfalls and solutions.
作者信息
Pelletier Mildred M, Kleinbongard Petra, Ringwood Lorna, Hito Rania, Hunter Christian J, Schechter Alan N, Gladwin Mark T, Dejam André
机构信息
Molecular Medicine Branch, NIDDK, National Institutes of Health, Bethesda, MD 20892, USA.
出版信息
Free Radic Biol Med. 2006 Aug 15;41(4):541-8. doi: 10.1016/j.freeradbiomed.2006.05.001. Epub 2006 May 10.
There are a number of difficulties involved in the quantification of nitrite in biological systems. These difficulties result from oxidation of nitrite (within minutes) by heme proteins, such as hemoglobin, myoglobin, cytoglobin, and neuroglobin; its low levels in vivo; and its ubiquitous presence in laboratory buffers and glassware. The goal of this review is to present an assay suitable for the sensitive and specific measurement of intravascular nitrite in mammals using the chemiluminescence-based nitric oxide analyzer and to inform the reader on how to evade the pitfalls pertinent to nitrite determination in biological matrices.
在生物系统中对亚硝酸盐进行定量分析存在诸多困难。这些困难源于亚硝酸盐（在数分钟内）会被血红素蛋白（如血红蛋白、肌红蛋白、细胞红蛋白和神经球蛋白）氧化；其在体内含量较低；以及它在实验室缓冲液和玻璃器皿中普遍存在。本综述的目的是介绍一种适用于使用基于化学发光的一氧化氮分析仪灵敏且特异测定哺乳动物血管内亚硝酸盐的检测方法，并告知读者如何规避生物基质中亚硝酸盐测定相关的陷阱。
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