Ragusa Maria A, Costa Salvatore, Cefalù Angelo B, Noto Davide, Fayer Francesca, Travali Salvatore, Averna Maurizio R, Gianguzza Fabrizio
Department of Cellular and Developmental Biology 'A. Monroy', University of Palermo, 90128 Palermo, Italy.
Int J Mol Med. 2006 Sep;18(3):449-55.
In this study, by using different techniques (i.e. Northern blot hybridization, RT-PCR and Southern blot hybridization) on various normal rat tissues, we were able to identify liver, kidney, heart, small intestine, brain, spleen, stomach and prostate as tissues in which the ApoH gene is transcribed. Moreover, for some of these tissues, by in situ hybridization, we found a specific localization of apoH transcripts. For instance epithelial cells of the bile ducts in liver and of the proximal tubules in kidney are the major sites of apoH synthesis. Our data suggest that some of the different physiological roles proposed for apoH could correlate with its direct expression, while others could correlate with its absorption from bloodstream or adjacent cells.
在本研究中，通过对各种正常大鼠组织运用不同技术（即Northern印迹杂交、逆转录-聚合酶链反应和Southern印迹杂交），我们能够确定肝脏、肾脏、心脏、小肠、大脑、脾脏、胃和前列腺为转录载脂蛋白H（ApoH）基因的组织。此外，对于其中一些组织，通过原位杂交，我们发现了载脂蛋白H转录本的特定定位。例如，肝脏中胆管的上皮细胞和肾脏中近端小管的上皮细胞是载脂蛋白H合成的主要部位。我们的数据表明，为载脂蛋白H提出的一些不同生理作用可能与其直接表达相关，而其他作用可能与其从血液或相邻细胞的吸收相关。
