Herías M Veronica, Hogenkamp Astrid, van Asten Alphons J A M, Tersteeg Monique H G, van Eijk Martin, Haagsman Henk P
Department of Infectious Diseases and Immunology, Division Molecular Host Defence, Graduate School of Animal Health, Faculty of Veterinary Medicine, Utrecht University, P.O. Box 80175, NL-3508 TD Utrecht, The Netherlands.
Mol Immunol. 2007 Jul;44(13):3324-32. doi: 10.1016/j.molimm.2007.02.025. Epub 2007 Apr 8.
Surfactant proteins A and D are pattern recognition molecules that play a role in pulmonary host defence. In this paper, we describe for the first time the expression and localisation of both collectins in various porcine tissues using a combination of in situ hybridisation (ISH), RT-PCR and immunohistochemistry (IHC). SP-D was expressed in several tissues including lung, tongue, intestinal tract, thymus, skin, gall bladder and lacrimal gland. Focal SP-D expression was detected in oesophagus, stomach, kidney, liver, prostate and spleen with both histological techniques. These tissues tested negative with RT-PCR. In contrast, SP-A expression was limited to the lung as measured by ISH and IHC. Interestingly, analysis by RT-PCR showed that thymus, trachea, jejunum and duodenum are positive for the presence of SP-A mRNA. We conclude that the combination of different methods can be advantageous if tissue-specific expression is studied. The importance of SP-D in innate immune defence of the pig is underlined by its expression at the potential ports of entry of pathogens.
表面活性蛋白A和D是模式识别分子，在肺部宿主防御中发挥作用。在本文中，我们首次结合原位杂交（ISH）、逆转录聚合酶链反应（RT-PCR）和免疫组织化学（IHC），描述了这两种凝集素在各种猪组织中的表达和定位。SP-D在包括肺、舌、肠道、胸腺、皮肤、胆囊和泪腺在内的多种组织中表达。通过两种组织学技术在食管、胃、肾、肝、前列腺和脾脏中检测到局灶性SP-D表达。这些组织经RT-PCR检测呈阴性。相比之下，通过ISH和IHC检测，SP-A的表达仅限于肺。有趣的是，RT-PCR分析表明胸腺、气管、空肠和十二指肠存在SP-A mRNA呈阳性。我们得出结论，如果研究组织特异性表达，不同方法的结合可能具有优势。SP-D在病原体潜在进入端口的表达突出了其在猪先天免疫防御中的重要性。
