Reuss Reinhard, Aberle Susanne, Klingel Karin, Sauter Martina, Greschniok Annette, Franke Folker Ernst, Padberg Winfried, Blin Nikolaus
Division of Molecular Genetics, Institute of Anthropology and Human Genetics, University Hospital Tübingen, Eberhard-Karls University Tübingen, D-72076 Tübingen, Germany.
Int J Oncol. 2006 Sep;29(3):649-54.
Since pancreatic cancer is an aggressive and often incurable malignancy, we investigated if the carboxyl ester lipase gene (CEL) is specifically expressed in pancreatic tissues and its promoter can be used for a specific suicide gene approach. Twenty-five tumor samples, 24 samples of normal pancreatic tissue and control tissues from other organs were examined by radioactive in situ hybridization (ISH) to localize CEL mRNA. Two carcinoma samples and 6 permanent cell lines were examined by reverse transcriptase-polymerase chain reaction (RT-PCR). By ISH, we verified a strong CEL gene expression in acinar cells of the normal pancreas. A minor expression was noted in a single sample of acinar cell carcinoma and adenocarcinomas did not show any expression. By RT-PCR, no specific expression in both tested adenocarcinomas was observed. In summary, these results show that, contrary to notable expression of carboxyl ester lipase in acinar cells of normal pancreatic tissue, this lipase is not significantly active in pancreatic adenocarcinomas and thus not an apt genetic marker for diagnostic or therapeutic approaches.
由于胰腺癌是一种侵袭性强且通常无法治愈的恶性肿瘤，我们研究了羧基酯脂肪酶基因（CEL）是否在胰腺组织中特异性表达，以及其启动子是否可用于特定的自杀基因方法。通过放射性原位杂交（ISH）对25个肿瘤样本、24个正常胰腺组织样本以及来自其他器官的对照组织进行检测，以定位CEL mRNA。通过逆转录聚合酶链反应（RT-PCR）对2个癌样本和6个永久细胞系进行检测。通过ISH，我们证实正常胰腺的腺泡细胞中有强烈的CEL基因表达。在单个腺泡细胞癌样本中观察到轻微表达，而腺癌未显示任何表达。通过RT-PCR，在两个测试的腺癌中均未观察到特异性表达。总之，这些结果表明，与羧基酯脂肪酶在正常胰腺组织腺泡细胞中的显著表达相反，这种脂肪酶在胰腺腺癌中没有显著活性，因此不是诊断或治疗方法的合适基因标志物。
