Kobayashi Michiko, Nagahisa Keisuke, Shimizu Hiroshi, Shioya Suteaki
Department of Biotechnology, Graduate School of Engineering, Osaka University, 2-1 Yamadaoka, Suita, 565-0871 Osaka, Japan.
Appl Microbiol Biotechnol. 2006 Dec;73(3):549-58. doi: 10.1007/s00253-006-0516-1. Epub 2006 Jul 25.
Volatile compounds cause undesirable flavor when their concentrations exceed threshold values in beer fermentation. The objective of this study is to develop a system for controlling apparent extract concentration, which indicates the fermentation degree and which should be decreased below a targeted value at a fixed time under a constraint of tolerable amounts of volatile compounds. In beer fermentation, even though the production of volatile compounds is suppressed by maintaining a low fermentation temperature, a low temperature causes a delay in the control of apparent extract concentration. Volatile compound concentration was estimated on-line, and the simulation of apparent extract consumption and volatile compound production was performed. To formulate various beer tastes and conserve energy for attemperation, optimal temperature profiles were determined using a genetic algorithm (GA). The developed feedback control of the brewing temperature profile was successfully applied, and apparent extract and volatile compound concentrations at a fixed time reached their target concentrations. Additionally, the control technique developed in this study enables us to brew a wide variety of beers with different tastes.
当挥发性化合物在啤酒发酵中的浓度超过阈值时,会产生不良风味。本研究的目的是开发一种用于控制表观浸出物浓度的系统,该浓度指示发酵程度,并且在挥发性化合物可接受量的限制下,应在固定时间降低至目标值以下。在啤酒发酵中,即使通过保持低温来抑制挥发性化合物的产生,但低温会导致表观浸出物浓度控制的延迟。在线估计挥发性化合物浓度,并对表观浸出物消耗和挥发性化合物产生进行模拟。为了调配出各种啤酒口味并节约调温能耗,使用遗传算法(GA)确定了最佳温度曲线。所开发的酿造温度曲线反馈控制得到成功应用,固定时间的表观浸出物和挥发性化合物浓度达到了目标浓度。此外,本研究中开发的控制技术使我们能够酿造出具有不同口味的多种啤酒。