Ogawa Ryoichiro, Saito Chikara, Jung Han-Sung, Ohshima Hayato
Division of Anatomy and Cell Biology of the Hard Tissue, Department of Tissue Regeneration and Reconstruction, Niigata University Graduate School of Medical and Dental Sciences, Niigata, Japan.
Cell Tissue Res. 2006 Dec;326(3):715-24. doi: 10.1007/s00441-006-0242-0. Epub 2006 Jul 25.
Under pathological conditions, dental pulp elaborates both bone and dentin matrix in which the contribution of periodontal tissue cannot be excluded. This study has aimed to clarify the capability of dental pulp to deposit bone matrix in an auto-graft experiment by using (1) immunohistochemistry for 5-bromo-2'-deoxyuridine (BrdU) and nestin and (2) histochemistry for tartrate-resistant acid phosphatase (TRAP). Following the extraction of the molars of 3-week-old mice, the roots and pulp floor were resected and immediately transplanted into the sublingual region. On Days 5-7, tubular dentin formation commenced next to the pre-existing dentin at the pulp horn in which nestin-positive odontoblast-like cells were arranged. Up until Day 14, bone-like tissue formation occurred in the pulp chamber in which intense TRAP-positive cells appeared. These results suggest that odontoblast- and osteoblast-lineage cells reside in the dental pulp. Overall, specific dental pulp regeneration should provide fundamental knowledge for the realization of human tooth regeneration in the near future.
在病理条件下，牙髓可形成骨和牙本质基质，其中牙周组织的作用也不能排除。本研究旨在通过（1）对5-溴-2'-脱氧尿苷（BrdU）和巢蛋白进行免疫组织化学检测，以及（2）对耐酒石酸酸性磷酸酶（TRAP）进行组织化学检测，在自体移植实验中阐明牙髓沉积骨基质的能力。在拔除3周龄小鼠的磨牙后，将牙根和牙髓底部切除并立即移植到舌下区域。在第5 - 7天，在牙髓角处靠近原有牙本质的位置开始形成管状牙本质，其中排列着巢蛋白阳性的成牙本质细胞样细胞。直到第14天，在牙髓腔中出现了骨样组织形成，其中有大量TRAP阳性细胞。这些结果表明，成牙本质细胞系和成骨细胞系细胞存在于牙髓中。总体而言，特定的牙髓再生应为在不久的将来实现人类牙齿再生提供基础知识。
