Tavokina L V, Sopko N I, Khazhilenko K G, Baronova E V
Tsitol Genet. 2006 Mar-Apr;40(2):72-8.
It is known that the frequency of chromosomal abnormalities among spontaneous miscarriages of the first trimester of pregnancy makes 50-60%. Research of karyotypes of chorionic villus cells of miscarriages has been conducted by combining the standard cytogenetic method and the FISH analysis on interphase nuclei of centromeric specific DNA samples by the tests to the chromosomes 13/21, 14/22, 15, 16, 18, X, Y. The described complex approach can be successfully applied for effective identification ofchromosomal abnormalities in the material of spontaneous miscarriages. The results specify the necessity of careful study of genomes of matrimonial pairs with the usual unmaturing in anamnesis and especially before treatment by IVF methods.
众所周知，妊娠头三个月自然流产中染色体异常的发生率为50-60%。通过将标准细胞遗传学方法与针对13/21、14/22、15、16、18、X、Y染色体的检测，对流产绒毛膜绒毛细胞的中期核进行荧光原位杂交（FISH）分析，开展了核型研究。所描述的这种综合方法可成功应用于有效识别自然流产材料中的染色体异常。结果表明，对于既往史中常有自然流产情况的配偶基因组，尤其是在采用体外受精方法治疗之前，有必要进行仔细研究。
