Ouchi E, Miura K, Yamagata S
Tohoku J Exp Med. 1975 Jun;116(2):141-8. doi: 10.1620/tjem.116.141.
The reticuloendothelial system function test using 131I-labeled aggregated human albumin (131IAA) as test particle is thought to be one of the best methods for the study of RES function of human beings. This method needs a large amount of 131IAA, but 131IAA has been used as a tracer of liver scintiscanning, and we tired to study RES function by using commercially available 131IAA specially made as tracer of liver scintiscanning. Because the amount of 131IAA used in this study was smaller than the critical dosis, our interest was focussed on the catabolic activity of 131IAA phagocytized by Kupffer's cells. The plasma obtained 60 min after 131IAA injection was fractionated into two distinct peaks by gel column chromatography; ionic 131I and protein-bound 131IAA. The ratio between ionic 131I and bound 131IAA (F/B ratio) was studied in various diseases. The F/B ratios of liver cirrhosis and hepatoma were significantly lower than that of the control. The lowered F/B ratio had no correlation with other liver function tests, data of hepatic scintiscanning, or with clinical findings. The F/B ratio was also not correlated with congo red index as RES function test.
以¹³¹I标记的聚合人血清白蛋白（¹³¹IAA）作为测试颗粒的网状内皮系统功能试验，被认为是研究人类网状内皮系统（RES）功能的最佳方法之一。该方法需要大量的¹³¹IAA，但¹³¹IAA已被用作肝脏闪烁扫描的示踪剂，我们试图通过使用专门作为肝脏闪烁扫描示踪剂的市售¹³¹IAA来研究RES功能。由于本研究中使用的¹³¹IAA量小于临界剂量，我们的兴趣集中在库普弗细胞吞噬的¹³¹IAA的分解代谢活性上。在注射¹³¹IAA 60分钟后获得的血浆通过凝胶柱色谱法分离为两个不同的峰；离子态¹³¹I和与蛋白质结合的¹³¹IAA。研究了各种疾病中离子态¹³¹I与结合的¹³¹IAA之间的比率（F/B比率）。肝硬化和肝癌的F/B比率显著低于对照组。降低的F/B比率与其他肝功能试验、肝脏闪烁扫描数据或临床表现均无相关性。F/B比率也与作为RES功能试验的刚果红指数无关。
