Morling N, Andersen V, Fugger L, Georgsen J, Halberg P, Oxholm P, Odum N, Svejgaard A
Tissue Typing Laboratory, State University Hospital, Rigshospitalet, Denmark.
Dis Markers. 1991 Sep-Oct;9(5):289-96.
We investigated the DNA restriction fragment length polymorphism (RFLP) of the Major Histocompatability Complex (MHC) class II genes: HLA-DRB, -DQA, -DQB, DPA, and -DFB in 24 patients with rheumatoid arthritis (RA), in 19 patients with primary Sjögren's syndrome (primary SS), and healthy Danes. The frequencies of DNA fragments associated with the following HLA class II genes were increased in RA when compared to normal controls: DRB104 (DR4) (relative risk, RR = 7.4, P less than 10(-3), DRB40101 (DRw53) (RR = 9.6, P less than 10(-3), DQA10301 (RR = 9.6, P less than 10(-3), DQB10301 (DQw7) (RR = 2.8, P less than 0.05, 'corrected' P greater than 0.05), and DQB10302 (DQw8) (RR = 4.5, P less than 10(-2). Negative associations were found between RA and DRB11501 (DR2/DRw15) (RR = 0.2, P less than 10(-2) and DQB10602 (DQw6) (RR = 0.2, P less than 10(-2), 'corrected' P greater than 0.05). The frequencies in RA of other HLA class II associated DNA fragments including DPA and DPB and the antigens DPw1-w6 defined by primed lymphocyte stimulation, did not differ significantly from those in controls. In primary SS, the frequency of HLA-B8 was significantly increased (RR = 9.0, P less than 10(-3). Positive associations were found between primary SS and DNA fragments associated with DRB103/13 (RR = 6.8, P less than 10(-3), DRB30101 (DRw52) (RR = 5.7, P less than 10(-2), DQA10501 (RR = 6.8, P less than 10(-3), DQB10201 (DQw2) (RR = 11.6, P less than 10(-5), and DQB10602 (DQw6) (RR = 2.7, P less than 0.05, 'corrected' P greater than 0.05).(ABSTRACT TRUNCATED AT 250 WORDS)
我们研究了24例类风湿性关节炎（RA）患者、19例原发性干燥综合征（原发性SS）患者及健康丹麦人主要组织相容性复合体（MHC）Ⅱ类基因：HLA - DRB、- DQA、- DQB、DPA和 - DFB的DNA限制性片段长度多态性（RFLP）。与正常对照相比，RA患者中与以下HLAⅡ类基因相关的DNA片段频率增加：DRB104（DR4）（相对风险，RR = 7.4，P小于10⁻³），DRB40101（DRw53）（RR = 9.6，P小于10⁻³），DQA10301（RR = 9.6，P小于10⁻³），DQB10301（DQw7）（RR = 2.8，P小于0.05，校正后P大于0.05），以及DQB10302（DQw8）（RR = 4.5，P小于10⁻²）。发现RA与DRB11501（DR2/DRw15）（RR = 0.2，P小于10⁻²）和DQB10602（DQw6）（RR = 0.2，P小于10⁻²，校正后P大于0.05）之间存在负相关。包括DPA和DPB以及通过预处理淋巴细胞刺激定义的抗原DPw1 - w6在内的其他HLAⅡ类相关DNA片段在RA中的频率与对照组无显著差异。在原发性SS中，HLA - B8的频率显著增加（RR = 9.0，P小于10⁻³）。发现原发性SS与与DRB103/13（RR = 6.8，P小于10⁻³）、DRB30101（DRw52）（RR = 5.7，P小于10⁻²）、DQA10501（RR = 6.8，P小于10⁻³）、DQB10201（DQw2）（RR = 11.6，P小于10⁻⁵）和DQB10602（DQw6）（RR = 2.7，P小于0.05，校正后P大于0.05）相关的DNA片段之间存在正相关。（摘要截断于250字）
