Determination of amoxycillin in plasma by ion pair column extraction and reversed-phase ion pair high-performance liquid chromatography.

作者信息

Jonkman J H, Schoenmaker R, Hempenius J

机构信息

Pharma Bio-Research Int., Laboratory for Drug Analysis and Biopharmaceutics, P.O. Box 147, 9400 AC Assen, The Netherlands; State University, Department of Pharmaceutical and Analytical Chemistry, Ant. Deusinglaan 2, 9713 AW Groningen, The Netherlands.

出版信息

J Pharm Biomed Anal. 1985;3(4):359-65. doi: 10.1016/0731-7085(85)80044-3.

DOI:10.1016/0731-7085(85)80044-3

PMID:16867672

Abstract

A quantitative method is described for the determination of amoxycillin in plasma. The method utilizes ion pair extraction of amoxycillin on disposable columns packed with Baker-10 SPE octadecyl, with tetrabutylammonium ion as the counter ion and methanol as the eluent. Separation and quantitation is performed by reversed-phase ion pair high-performance liquid chromatography (Nucleosil C-18) using the same counter ion and a mobile phase of methanol-phosphate buffer (pH 6.0) (31:69 v/v) with detection at 229 nm.

摘要