Buggé C J, Rodriguez L C, Vane F M
Department of Pharmacokinetics, Biopharmaceutics and Drug Metabolism, Roche Research Center, Hoffmann-La Roche Inc., Nutley, NJ 07110, USA.
J Pharm Biomed Anal. 1985;3(3):269-77. doi: 10.1016/0731-7085(85)80032-7.
A method is described for the quantitative analysis of isotretinoin and its 4-oxo metabolite, or of etretinate and its principal metabolites, in human blood in the range 10-2000 ng/ml. Following a simple one-step extraction, the compounds are determined by reversed-phase high-performance liquid chromatography (HPLC) with gradient elution and detection at 365 nm. This highly specific method separates the cis and trans isomers of the parent compounds and their metabolites. Examples are given of the application of this method to clinical studies of these two therapeutically important retinoids.
本文描述了一种用于定量分析人血液中异维A酸及其4-氧代代谢物,或阿维A酯及其主要代谢物的方法,检测范围为10-2000 ng/ml。经过简单的一步萃取后,采用梯度洗脱和365 nm检测的反相高效液相色谱法(HPLC)测定这些化合物。这种高特异性方法可分离母体化合物及其代谢物的顺式和反式异构体。文中给出了该方法应用于这两种具有重要治疗意义的维甲酸临床研究的实例。