Luvoni G C, Chigioni S, Beccaglia M
Department of Veterinary Clinical Sciences, Obstetrics and Gynaecology, University of Milan, Milan, Italy.
Reprod Domest Anim. 2006 Aug;41(4):286-90. doi: 10.1111/j.1439-0531.2006.00704.x.
Increased availability of canine embryos would be desirable to develop research and to apply assisted reproductive technologies in the treatment of infertility and in the improvement of reproductive performances in valuable Canids, both domestic and non-domestic. Embryo production through in vitro fertilization and nuclear transfer has been technically achieved in the dog, and the transfer of cloned embryos has recently resulted in the birth of puppies. However, the efficiency of these technologies is still very limited. This is mainly because of the peculiar characteristics of the canine oocyte and the lack of its full acquisition of developmental competence in vitro. This paper discusses the latest results and aspects on which further research should be focused to provide advances in the field.
增加犬类胚胎的可获得性，对于开展研究以及应用辅助生殖技术治疗不育症和改善家养及非家养珍贵犬科动物的繁殖性能而言是十分必要的。通过体外受精和核移植生产胚胎在技术上已在犬类中实现，并且克隆胚胎的移植最近已导致幼犬诞生。然而，这些技术的效率仍然非常有限。这主要是由于犬类卵母细胞的特殊特性以及其在体外未能充分获得发育能力。本文讨论了最新成果以及该领域进一步研究应聚焦的方面，以期取得进展。
