Boutros Michael, Brás Lígia P, Huber Wolfgang
Signaling and Functional Genomics, German Cancer Research Center, Im Neuenheimer Feld 580, 69120 Heidelberg, Germany.
Genome Biol. 2006;7(7):R66. doi: 10.1186/gb-2006-7-7-R66.
RNA interference (RNAi) screening is a powerful technology for functional characterization of biological pathways. Interpretation of RNAi screens requires computational and statistical analysis techniques. We describe a method that integrates all steps to generate a scored phenotype list from raw data. It is implemented in an open-source Bioconductor/R package, cellHTS (http://www.dkfz.de/signaling/cellHTS). The method is useful for the analysis and documentation of individual RNAi screens. Moreover, it is a prerequisite for the integration of multiple experiments.
RNA干扰(RNAi)筛选是一种用于生物途径功能表征的强大技术。RNAi筛选的解读需要计算和统计分析技术。我们描述了一种将所有步骤整合起来以从原始数据生成评分表型列表的方法。它在一个开源的生物导体/R包cellHTS(http://www.dkfz.de/signaling/cellHTS)中实现。该方法对于单个RNAi筛选的分析和记录很有用。此外,它是整合多个实验的先决条件。