RNAither，一种用于高通量RNA干扰筛选统计分析的自动化流程。
RNAither, an automated pipeline for the statistical analysis of high-throughput RNAi screens.
作者信息
Rieber Nora, Knapp Bettina, Eils Roland, Kaderali Lars
机构信息
Viroquant Research Group Modeling, University of Heidelberg, Bioquant BQ26, Im Neuenheimer Feld 267, 69120 Heidelberg, Germany.
出版信息
Bioinformatics. 2009 Mar 1;25(5):678-9. doi: 10.1093/bioinformatics/btp014. Epub 2009 Jan 25.
SUMMARY
We present RNAither, a package for the free statistical environment R which performs an analysis of high-throughput RNA interference (RNAi) knock-down experiments, generating lists of relevant genes and pathways out of raw experimental data. The library provides a quality assessment of the signal intensities, as well as a broad range of options for data normalization, different statistical tests for the identification of significant siRNAs, and a significance analysis of the biological processes involving corresponding genes. The results of the analysis are presented as a set of HTML pages. Additionally, all values and plots are available as either text files or pdf and png files.
AVAILABILITY
摘要
我们展示了RNAither，这是一个用于免费统计环境R的软件包，它对高通量RNA干扰（RNAi）敲低实验进行分析，从原始实验数据中生成相关基因和通路列表。该库提供信号强度的质量评估，以及数据归一化的多种选项、用于识别显著小干扰RNA（siRNA）的不同统计测试，以及对涉及相应基因的生物学过程的显著性分析。分析结果以一组HTML页面呈现。此外，所有值和图均可作为文本文件或pdf和png文件获取。
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