Rieber Nora, Knapp Bettina, Eils Roland, Kaderali Lars
Viroquant Research Group Modeling, University of Heidelberg, Bioquant BQ26, Im Neuenheimer Feld 267, 69120 Heidelberg, Germany.
Bioinformatics. 2009 Mar 1;25(5):678-9. doi: 10.1093/bioinformatics/btp014. Epub 2009 Jan 25.
We present RNAither, a package for the free statistical environment R which performs an analysis of high-throughput RNA interference (RNAi) knock-down experiments, generating lists of relevant genes and pathways out of raw experimental data. The library provides a quality assessment of the signal intensities, as well as a broad range of options for data normalization, different statistical tests for the identification of significant siRNAs, and a significance analysis of the biological processes involving corresponding genes. The results of the analysis are presented as a set of HTML pages. Additionally, all values and plots are available as either text files or pdf and png files.
我们展示了RNAither，这是一个用于免费统计环境R的软件包，它对高通量RNA干扰（RNAi）敲低实验进行分析，从原始实验数据中生成相关基因和通路列表。该库提供信号强度的质量评估，以及数据归一化的多种选项、用于识别显著小干扰RNA（siRNA）的不同统计测试，以及对涉及相应基因的生物学过程的显著性分析。分析结果以一组HTML页面呈现。此外，所有值和图均可作为文本文件或pdf和png文件获取。
