Scheuermann D W, Adriaensen D, Timmermans J P, De Groodt-Lasseel M H
Institute of Histology and Microscopic Anatomy, University of Antwerpen, Belgium.
Acta Histochem. 1991;91(2):185-92. doi: 10.1016/S0065-1281(11)80274-6.
Light microscopical immunohistochemistry was used to demonstrate the regulatory peptides present in the endocrine pancreas of Protopterus aethiopicus. The peptides studied included insulin, glucagon, pancreatic polypeptide and somatostatin. The results showed that the 4 regulatory peptides commonly detected in the mammalian endocrine pancreas were immunologically discernible in this dipnoan fish. Large amounts of insulin-immunoreactive cells, in the centre of the pancreatic islets, were surrounded by a small rim of glucagon-or pancreatic polypeptide-immunoreactive cells. In addition, adjacent sections stained with anti-glucagon and anti-pancreatic polypeptide revealed that these hormones could be found in the same cells. Somatostatin-positive cells were scattered throughout the islets. Their processes were seen to contact many different endocrine pancreatic cells, suggesting that the somatostatin-immunoreactive cells control the functions of other endocrine pancreatic cells.
采用光学显微镜免疫组织化学方法来显示非洲肺鱼内分泌胰腺中存在的调节肽。所研究的肽包括胰岛素、胰高血糖素、胰多肽和生长抑素。结果表明，在哺乳动物内分泌胰腺中通常检测到的这4种调节肽在这种肺鱼中在免疫方面是可辨别的。胰岛中央大量的胰岛素免疫反应细胞被一小圈胰高血糖素或胰多肽免疫反应细胞所包围。此外，用抗胰高血糖素和抗胰多肽染色的相邻切片显示，这些激素可存在于同一细胞中。生长抑素阳性细胞散布于整个胰岛。可见它们的突起与许多不同的内分泌胰腺细胞接触，这表明生长抑素免疫反应细胞控制着其他内分泌胰腺细胞的功能。
