Leigh C M, Edwin N
Department of Anatomy, University of Adelaide, South Australia.
Eur J Histochem. 1997;41(3):217-21.
The endocrine pancreas of the southern hairy-nosed wombat (Lasiorhinus latifrons) was investigated by means of electron-microscope immunocytochemistry using the protein A--gold technique on London resin (LR) white-embedded tissue. The primary antibodies used were raised against insulin, glucagon, somatostatin and pancreatic polypeptide. The morphology of the secretory granules differed in the four cell types. The insulin cells are pleiomorphic, and the secretory granules composed of an electron-dense core surrounded by an electron-lucent halo. The glucagon cells possess granules of varying density. Somatostatin cells have large, less dense secretory granules. The pancreatic polypeptide cells show small dense secretory granules. It is essential that it be corroborated by immunocytochemical data at the light or preferably electron-microscopical level for the definite identification of endocrine cell types. Recent developments in immuno-electron-microscopic techniques have contributed to a better knowledge of cells responsible for the secretion of a wide variety of hormones, as in this study.
利用蛋白A-金技术对伦敦树脂（LR）白色包埋组织进行电子显微镜免疫细胞化学研究，对南方毛鼻袋熊（Lasiorhinus latifrons）的内分泌胰腺进行了研究。所用的一抗是针对胰岛素、胰高血糖素、生长抑素和胰多肽制备的。四种细胞类型中分泌颗粒的形态各不相同。胰岛素细胞形态多样，分泌颗粒由电子致密核心和周围的电子透亮晕组成。胰高血糖素细胞拥有密度各异的颗粒。生长抑素细胞有大的、密度较小的分泌颗粒。胰多肽细胞显示出小的致密分泌颗粒。为了明确鉴定内分泌细胞类型，必须通过光镜或更理想的电镜水平的免疫细胞化学数据加以证实。免疫电子显微镜技术的最新进展有助于更好地了解负责分泌多种激素的细胞，本研究即是如此。
