Mercadal C M, Martin S, Rouse B T
Department of Microbiology, University of Tennessee, Knoxville.
Viral Immunol. 1991 Fall;4(3):177-86. doi: 10.1089/vim.1991.4.177.
Mice depleted in vivo of either CD4+ or CD8+ T cells were used to define the requirement for interaction between the two T subsets for the induction and maturation of a herpes simplex virus (HSV) cytotoxic T-lymphocyte (CTL) response. Whereas C3H mice generated normal CD8+ CTL in the absence of CD4+ T cells, responses were undetectable in BALB/c mice. However, the role of CD4+ T cells appeared to be to supply helper factors for CTL maturation, as the numbers of CTL precursors in CD4-depleted mice were similar to those in nondepleted animals. Moreover, CTL responses were demonstrable if CD4-depleted primed populations were stimulated with antigen or supplied with a source of helper factors. The optimal means of presenting antigen appeared to be via dendritic cells. Our results indicated that CD8+ cells alone were fully capable of differentiating into CTL provided they were appropriately stimulated with antigen. Possibly, the environment necessary for this to occur in vivo is usually lacking, accounting for the fact that in the mouse, it usually is not possible to demonstrate HSV-specific CTL unless cells are cultured or restimulated in vitro.
利用体内耗竭CD4⁺或CD8⁺T细胞的小鼠来确定两种T细胞亚群之间相互作用对于单纯疱疹病毒（HSV）细胞毒性T淋巴细胞（CTL）反应的诱导和成熟的必要性。在缺乏CD4⁺T细胞的情况下，C3H小鼠能产生正常的CD8⁺CTL，但在BALB/c小鼠中检测不到反应。然而，CD4⁺T细胞的作用似乎是为CTL成熟提供辅助因子，因为CD4⁺细胞耗竭小鼠中的CTL前体细胞数量与未耗竭动物中的相似。此外，如果用抗原刺激CD4⁺细胞耗竭的致敏群体或为其提供辅助因子来源，CTL反应是可以证明的。呈现抗原的最佳方式似乎是通过树突状细胞。我们的结果表明，只要用抗原进行适当刺激，单独的CD8⁺细胞完全能够分化为CTL。可能在体内发生这种情况所需的环境通常不存在，这就解释了在小鼠中，除非在体外培养或再次刺激细胞，否则通常无法证明存在HSV特异性CTL这一事实。
