Webster Paul
Ahmanson Center for Advanced Electron Microscopy & Imaging, House Ear Institute, Los Angeles, California 90057, USA.
Autophagy. 2006 Jul-Sep;2(3):159-61. doi: 10.4161/auto.2826. Epub 2006 Jul 9.
Cytoplasmic bacteria may assist in our study of the autophagic pathway. This review highlights the use of Listeria monocytogenes for examining the assembly of autophagic vacuoles in mammalian cells. Inhibiting protein synthesis of cytoplasmic L. monocytogenes results in their being sequestered into the autophagic pathway. Autophagic vacuoles form around the easily identified bacterial particles making the assembly process easy to study using morphological and biochemical methods. L. monocytogenes, which appears to be ideally adapted to life in the cell cytoplasm, does not normally become a target of autophagy. In model systems the bacteria thrive within host cell cytoplasm, indicating the importance of de novo protein synthesis in avoiding the autophagic pathway. This observation indicates an interesting opportunity for identifying the bacterial mechanisms that are mobilized to avoid the autophagic pathway.
细胞质细菌可能有助于我们对自噬途径的研究。本综述着重介绍了利用单核细胞增生李斯特菌来研究哺乳动物细胞中自噬泡的组装。抑制细胞质中的单核细胞增生李斯特菌的蛋白质合成会导致它们被隔离到自噬途径中。自噬泡围绕易于识别的细菌颗粒形成，使得利用形态学和生物化学方法来研究组装过程变得容易。单核细胞增生李斯特菌似乎非常适应在细胞质中生存，通常不会成为自噬的目标。在模型系统中，这种细菌在宿主细胞质中大量繁殖，这表明从头合成蛋白质对于避免进入自噬途径的重要性。这一观察结果为识别被调动以避免自噬途径的细菌机制提供了一个有趣的机会。
