Vassileva V, Galabov A
Acta Microbiol Acad Sci Hung. 1975;22(3):323-8.
The production and effect of interferon in the virus-transformed cell line TGk1, originating from kidney cells of Testudo gracea were studied and compared to those in the primary cell culture. West Nile virus and Newcastle disease virus were used as inducers. Interferon production in TGk1 cells began 6 hr later than in the primary cell culture and reached the maximum 64 IU, 18 hr after virus inoculation. In the primary culture, interferon production increased till the 48th hr reaching a fourfold level (256 IU). A significant reduction of the antiviral effect of interferon against vesicular stomatitis virus but not against vaccinia virus was observed in the transformed cells. The decreased interferon production and effect in TGk1 cells is regarded as a consequence of the disturbance of the interferon regulatory mechanism taking place as a result of the virus-induced transformation.
研究了源自希腊陆龟肾细胞的病毒转化细胞系TGk1中干扰素的产生及作用，并与原代细胞培养中的情况进行了比较。使用西尼罗河病毒和新城疫病毒作为诱导剂。TGk1细胞中干扰素的产生比原代细胞培养晚6小时开始，在病毒接种后18小时达到最大值64国际单位。在原代培养中，干扰素产生一直增加到第48小时，达到四倍水平（256国际单位）。在转化细胞中观察到干扰素对水疱性口炎病毒的抗病毒作用显著降低，但对痘苗病毒没有影响。TGk1细胞中干扰素产生和作用的降低被认为是病毒诱导转化导致干扰素调节机制紊乱的结果。
