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硝化杆菌属中norX/nxrX正确起始密码子的验证及norAXB/nxrAXB基因簇的通用性

Validation of the correct start codon of norX/nxrX and universality of the norAXB/nxrAXB gene cluster in nitrobacter species.

作者信息

Vanparys B, Bodelier P, De Vos P

机构信息

Laboratory of Microbiology, Department of Biochemistry, Physiology and Microbiology, Ghent University, K. L. Ledeganckstraat 35, B-9000 Gent, Belgium.

出版信息

Curr Microbiol. 2006 Sep;53(3):255-7. doi: 10.1007/s00284-006-0161-z. Epub 2006 Jul 27.

Abstract

The complete norX/nxrX sequence of five Nitrobacter strains was determined showing that the norAXB/nxrAXB gene cluster is present in all hitherto described Nitrobacter species. Evidence is provided that the previously published sequence of norX in N. hamburgensis X14(T) contains an invalid base "insertion," which resulted in a frameshift and a misidentified start codon.

摘要

测定了五株硝化杆菌菌株的完整norX/nxrX序列,结果表明norAXB/nxrAXB基因簇存在于所有迄今已描述的硝化杆菌物种中。有证据表明,先前发表的汉堡硝化杆菌X14(T)中norX的序列包含一个无效碱基“插入”,这导致了移码突变和起始密码子的错误识别。

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