Takamatsu Y, Yamamoto H, Ogunremi O O, Matsuzaki I, Moroji T
Department of Psychopharmacology, Psychiatric Research Institute of Tokyo, Japan.
Neuropeptides. 1991 Dec;20(4):255-65. doi: 10.1016/0143-4179(91)90016-c.
Our previous study showed that intracerebroventricular (ICV) administration of corticotropin-releasing hormone (CRH) produced a significant increase in locomotor activity at a dose of 1 microgram and slow stereotypy with prominent grooming at a dose of 10 micrograms. In addition, the ICV administration of CRH caused a significant increase in dopamine (DA) and norepinephrine turnover (NE) in various forebrain regions. The present study was designed to investigate the effects of the ICV administration of CRH on cholecystokinin (CCK), neuropeptide Y (NPY), somatostatin (SOM) and gamma-amino butyric acid (GABA) in the rat forebrain. The ICV administration of 1 and 10 micrograms CRH caused a marked reduction in CCK-like immunoreactivity (CCK-LI), NPY-LI and SOM-LI in the medial frontal cortex (MFC) and anterior cingulate cortex (Ant.CC), whereas it induced an increment of NPY-LI in the nucleus accumbens (NAc) and striatum. Increased SOM-LI and decreased NPY-LI were observed in the hippocampus following the ICV administration of CRH at both doses. The ICV administration of CRH caused a significant decrease in the BAGA content in the MFC, ant.CC, NAc and striatum. Taken together with our previous findings, these results indicate that the ICV administration of CRH induced classical neurotransmitter and neuropeptide abnormalities in the central nervous system which resulted increased emotionality, especially anxiety, in rats.
我们之前的研究表明，脑室内（ICV）注射促肾上腺皮质激素释放激素（CRH），剂量为1微克时会显著增加运动活性，剂量为10微克时会出现缓慢的刻板行为并伴有明显的梳理行为。此外，脑室内注射CRH会导致各个前脑区域的多巴胺（DA）和去甲肾上腺素周转率（NE）显著增加。本研究旨在探讨脑室内注射CRH对大鼠前脑胆囊收缩素（CCK）、神经肽Y（NPY）、生长抑素（SOM）和γ-氨基丁酸（GABA）的影响。脑室内注射1微克和10微克CRH会导致内侧额叶皮质（MFC）和前扣带回皮质（Ant.CC）中CCK样免疫反应性（CCK-LI）、NPY-LI和SOM-LI显著降低，而在伏隔核（NAc）和纹状体中会诱导NPY-LI增加。两种剂量的CRH脑室内注射后，海马体中观察到SOM-LI增加和NPY-LI降低。脑室内注射CRH会导致MFC、Ant.CC、NAc和纹状体中GABA含量显著降低。结合我们之前的研究结果，这些结果表明，脑室内注射CRH会在中枢神经系统中诱导经典神经递质和神经肽异常，从而导致大鼠情绪增加，尤其是焦虑情绪。
