Kowalski C, Micheau J, Corder R, Gaillard R, Conte-Devolx B
Laboratoire de Neuroendrocinologie Expérimentale, INSERM U 297, Faculté de Médecine Nord, Marseille, France.
Brain Res. 1992 Jun 5;582(1):38-46. doi: 10.1016/0006-8993(92)90314-y.
We investigated the age-related changes in the tissular protein, cortico-releasing factor (CRF), somatostatin (SOM), neuropeptide Y(NPY), methionine enkephalin (M-ENK) and beta-endorphin (beta-END) levels in frontal cortex, hippocampus, striatum and hypothalamus of young (4-month-old), mature (18-month-old) and senescent (26-month-old) Wistar male rats, bred in a specific pathogen free environment. Between the age of 4 and 18 months, the tissular protein levels increased in all 4 structures studied. The CRF and SOM levels increased in the hippocampus, while the NPY levels decreased. During this time, the NPY content increased in the striatum, whereas the SOM and M-Enk striatal levels decreased. Concomitantly, the NPY and beta-End levels decreased in the hypothalamus. Interestingly, no significant variations were found to occur in the frontal cortex whatever the neuropeptide studied. Between the age of 18 and 26 months, no significant changes in the tissular protein levels were detected, except in the hippocampus. The changes in the neuropeptide concentrations observed during this period depended on the neuropeptide and the brain structure studied. The CRF and beta-End levels decreased in the frontal cortex and the hypothalamus, respectively. The NPY peptidergic systems seem to be preferentially affected by aging processes since 3 out of the 4 structures studied--the frontal cortex, the striatum and the hypothalamus--showed a decrease in their tissular NPY content. During the same period, none of the 5 neuropeptides studied were affected in the hippocampus.
我们研究了在无特定病原体环境中饲养的年轻（4 个月大）、成熟（18 个月大）和衰老（26 个月大）雄性 Wistar 大鼠额叶皮质、海马体、纹状体和下丘脑组织中蛋白质、促肾上腺皮质激素释放因子（CRF）、生长抑素（SOM）、神经肽 Y（NPY）、甲硫氨酸脑啡肽（M-ENK）和β-内啡肽（β-END）水平的年龄相关变化。在 4 至 18 个月龄之间，所研究的所有 4 个结构中的组织蛋白质水平均升高。海马体中 CRF 和 SOM 水平升高，而 NPY 水平降低。在此期间，纹状体中 NPY 含量增加，而纹状体中 SOM 和 M-脑啡肽水平降低。同时，下丘脑中 NPY 和β-内啡肽水平降低。有趣的是，无论研究哪种神经肽，额叶皮质均未发现明显变化。在 18 至 26 个月龄之间，除海马体外，未检测到组织蛋白质水平有明显变化。在此期间观察到的神经肽浓度变化取决于所研究的神经肽和脑结构。额叶皮质和下丘脑中的 CRF 和β-内啡肽水平分别降低。NPY 肽能系统似乎优先受到衰老过程的影响，因为所研究的 4 个结构中的 3 个——额叶皮质、纹状体和下丘脑——其组织中 NPY 含量均降低。在同一时期，所研究的 5 种神经肽在海马体中均未受到影响。
