从肝吸虫（肝片吸虫）中分离出一种胰多肽样神经肽并进行部分测序。

Isolation and partial sequencing of a pancreatic polypeptide-like neuropeptide from the liver fluke, Fasciola hepatica.

Magee R M, Shaw C, Fairweather I, Thim L, Johnston C F, Halton D W

Comparative Neuroendocrinology Research Group, School of Biology, Queen's University of Belfast, Northern Ireland.

Comp Biochem Physiol C Comp Pharmacol Toxicol. 1991;100(3):507-11. doi: 10.1016/0742-8413(91)90031-n.

Abstract

A pancreatic polypeptide (PP)-immunoreactive neuropeptide has been isolated and partially sequenced from the liver fluke, Fasciola hepatica. 2. Gel permeation chromatography of an acid ethanol extract of cattle flukes showed that the peptide is similar in size to mammalian (bovine) PP. 3. The Fasciola peptide was purified to homogeneity by means of reverse-phase HPLC, employing different column chemistries. 4. The purified peptide was sequenced using automated gas-phase Edman degradation and the first 24 amino acid residues determined.

摘要

已从肝片吸虫（Fasciola hepatica）中分离出一种胰腺多肽（PP）免疫反应性神经肽，并对其进行了部分测序。2. 牛吸虫酸性乙醇提取物的凝胶渗透色谱分析表明，该肽的大小与哺乳动物（牛）的PP相似。3. 利用反相高效液相色谱法，采用不同的柱化学方法，将肝片吸虫肽纯化至同质。4. 使用自动气相Edman降解法对纯化后的肽进行测序，并确定了前24个氨基酸残基。