Yamada K, Sakai M, Takeuchi T, Karasawa N, Kaneda N, Sasaoka T, Kobayashi K, Yokoyama M, Nomura T, Katsuki M
Department of Anatomy, School of Medicine, Fujita Health University, Toyoake, Japan.
Neurosci Lett. 1991 Dec 16;134(1):57-61. doi: 10.1016/0304-3940(91)90508-q.
We have previously reported the distribution of human tyrosine hydroxylase (TH) transgene expression in dopaminergic neurons (ventral tegmental area and substantia nigra), adrenal gland, and non-catecholaminergic neurons in the forebrain of transgenic (Tg) mice. In this paper, we analysed the transgene expression in catecholaminergic (CAergic) neurons in the lower brainstem of Tg mice, by in situ hybridization and immunocytochemistry at the light and electron microscopic levels. High-level hybridization signals of the human TH mRNA were observed in the locus ceruleus and nucleus tractus solitarii of the Tg brain. Intense TH immunoreactivity was expressed specifically in the Tg brainstem, as was observed in non-Tg mice. These results reveal that the human TH transgene contains the regulatory elements responsible for the expression in three kinds of CAergic (dopaminergic, noradrenergic and adrenergic) neurons of the mouse brain.
我们之前报道了人类酪氨酸羟化酶（TH）转基因在转基因（Tg）小鼠中多巴胺能神经元（腹侧被盖区和黑质）、肾上腺以及前脑非儿茶酚胺能神经元中的表达分布。在本文中，我们通过光镜和电镜水平的原位杂交及免疫细胞化学方法，分析了Tg小鼠脑桥中儿茶酚胺能（CAergic）神经元中的转基因表达情况。在Tg脑的蓝斑和孤束核中观察到人类TH mRNA的高水平杂交信号。与非Tg小鼠一样，强烈的TH免疫反应性在Tg脑干中特异性表达。这些结果表明，人类TH转基因包含负责在小鼠脑的三种CAergic（多巴胺能、去甲肾上腺素能和肾上腺素能）神经元中表达的调控元件。
