Nagatsu I, Yamada K, Karasawa N, Sakai M, Takeuchi T, Kaneda N, Sasaoka T, Kobayashi K, Yokoyama M, Nomura T
Department of Anatomy, School of Medicine, Fujita Health University, Toyoake, Japan.
Neurosci Lett. 1991 Jun 10;127(1):91-5. doi: 10.1016/0304-3940(91)90902-6.
We have recently reported the production of transgenic (Tg) mice carrying the human tyrosine hydroxylase (TH) gene, and have described tissue-specific expression of the transgene in catecholaminergic (CAergic) neurons and adrenal glands. This paper describes the transgene expression in non-catecholaminergic (nCAergic) neurons in the brain of Tg mice by immunocytochemistry and in situ hybridization. In adult Tg mice, human TH was atypically expressed in the olfactory (typically, the anterior olfactory nucleus and pyriform cortex) and visual (typically, n. suprachiasmaticus and n. parabigeminalis) systems, in addition to typical CAergic neuron-rich nuclei in the brain. These results suggest the possibility that TH plays some novel roles in sensory systems.
我们最近报道了携带人类酪氨酸羟化酶（TH）基因的转基因（Tg）小鼠的产生，并描述了该转基因在儿茶酚胺能（CAergic）神经元和肾上腺中的组织特异性表达。本文通过免疫细胞化学和原位杂交描述了Tg小鼠脑中非儿茶酚胺能（nCAergic）神经元中的转基因表达。在成年Tg小鼠中，除了脑中典型的富含CAergic神经元的核团外，人类TH还在嗅觉系统（典型的是前嗅核和梨状皮质）和视觉系统（典型的是视交叉上核和副视束核）中异常表达。这些结果提示TH在感觉系统中可能发挥一些新作用。
