Chantry-Darmon C, Urien C, de Rochambeau H, Allain D, Pena B, Hayes H, Grohs C, Cribiu E P, Deretz-Picoulet S, Larzul C, Save J C, Neau A, Chardon P, Rogel-Gaillard C
Laboratoire de Radiobiologie et Etude du Génome, UMR INRA CEA 314, Domaine de Vilvert, 78350 Jouy-en-Josas, France.
Anim Genet. 2006 Aug;37(4):335-41. doi: 10.1111/j.1365-2052.2006.01462.x.
Although the European rabbit (Oryctolagus cuniculus) is used both in agronomics and in research, genomic resources for this species are still limited and no microsatellite-based genetic map has been reported. Our aim was to construct a rabbit genetic map with cytogenetically mapped microsatellites so as to build an integrated genetic and cytogenetic map. A reference population of 187 rabbits comprising eight three-generation families with 10-25 offspring per family was produced. One hundred and ninety-four of 305 previously identified microsatellites were included in this study. Of these, 158 were polymorphic with two to seven alleles. The map reported here comprises 111 markers, including 104 INRA microsatellites, five microsatellites from another source and two phenotypic markers (angora and albino). Ninety markers were integrated into 20 linkage groups. The remaining 21 microsatellites mapped to separate linkage groups, 19 with a precise cytogenetic position and two with only a chromosomal assignment. The genetic map spans 2766.6 cM and covers 20 rabbit chromosomes, excluding chromosomes 20, 21 and X. The density of this map is limited, but we used it to verify the location of angora and albino on chromosomes 15q and 1q, respectively, in agreement with previously published data. This first generation genetic/cytogenetic map will help gene identification and quantitative trait loci mapping projects in rabbit.
尽管欧洲兔（穴兔）在农学和研究中都有应用，但该物种的基因组资源仍然有限，尚未有基于微卫星的遗传图谱报道。我们的目标是构建一个带有细胞遗传学定位微卫星的兔遗传图谱，以建立一个整合的遗传和细胞遗传图谱。我们构建了一个由187只兔子组成的参考群体，包括8个三代家系，每个家系有10 - 25个后代。本研究纳入了先前鉴定的305个微卫星中的194个。其中，158个具有多态性，有2至7个等位基因。这里报道的图谱包含111个标记，包括104个法国国家农业研究院（INRA）微卫星、5个来自其他来源的微卫星和2个表型标记（安哥拉和白化）。90个标记整合到20个连锁群中。其余21个微卫星定位到单独的连锁群，其中19个有精确的细胞遗传学位置，2个只有染色体定位。遗传图谱跨度为2766.6厘摩，覆盖20条兔染色体，不包括20号、21号染色体和X染色体。该图谱的密度有限，但我们利用它验证了安哥拉和白化基因分别位于15q和1q染色体上，这与先前发表的数据一致。这第一代遗传/细胞遗传图谱将有助于兔的基因鉴定和数量性状位点定位项目。
