Myers-Evert Dawn K, Herrmann-Hoesing Lynn M
Washington State University, College of Veterinary Medicine, Pullman, WA 99164, USA.
J Virol Methods. 2006 Nov;137(2):339-42. doi: 10.1016/j.jviromet.2006.06.025. Epub 2006 Aug 1.
A Western blot assay was developed and analyzed against the comparable standard, immunoprecipitation of (35)[S]-methionine/cysteine-labeled ovine progressive pneumonia virus (OPPV) proteins, for its ability to detect anti-OPPV antibodies using endpoint titers. Western blot analysis is 12-fold more sensitive in detecting endpoint anti-capsid antibody titers than IP, and the capsid is the B-cell immunodominant OPPV protein when utilizing Western blot analysis. Since the surface envelope glycoprotein is the B-cell immunodominant OPPV protein when utilizing immunoprecipitation, this suggests immunoprecipitation and Western blot analysis measure different types of antibody that are more specific for conformational and linear OPPV protein epitopes, respectively.
开发了一种蛋白质免疫印迹分析方法，并与可比标准品进行分析，即对用（35）[S]-甲硫氨酸/半胱氨酸标记的绵羊进行性肺炎病毒（OPPV）蛋白进行免疫沉淀，以检测其使用终点效价检测抗OPPV抗体的能力。蛋白质免疫印迹分析在检测终点抗衣壳抗体效价方面比免疫沉淀灵敏12倍，并且在使用蛋白质免疫印迹分析时，衣壳是B细胞免疫显性的OPPV蛋白。由于在使用免疫沉淀时，表面包膜糖蛋白是B细胞免疫显性的OPPV蛋白，这表明免疫沉淀和蛋白质免疫印迹分析分别测量了对构象性和线性OPPV蛋白表位更具特异性的不同类型抗体。
