利用高效液相色谱指纹图谱法鉴别虎杖与近缘药材。
Differentiation of rhizoma et radix polygoni cuspidati from closely related herbs by HPLC fingerprinting.
作者信息
Qian Guangsheng, Leung Sik-Yuen, Lu GuangHua, Leung Kelvin Sze-Yin
机构信息
Research and Development Division, School of Chinese Medicine, Hong Kong Baptist University, Kowloon Tong, Kowloon, Hong Kong Special Administrative Region, P.R. China.
出版信息
Chem Pharm Bull (Tokyo). 2006 Aug;54(8):1179-86. doi: 10.1248/cpb.54.1179.
An HPLC-DAD fingerprinting profile of Rhizoma Et Radix Polygoni Cuspidati was established basing on the consistent chromatographic features of 24 authentic herb samples. The major types of chemical constituents, stilbenes and anthraquinones, were analyzed and included in the fingerprint. Eight common peaks of Polygonum Cuspidatum were identified by using HPLC-MS. The developed fingerprint was applied to differentiate Rhizoma Et Radix Polygoni Cuspidati from Radix Polygoni Multiflori and Radix Et Rhizoma Rhei. Although the three herbs belong to the family of Polygonaceae, the results indicated that these could be differentiated by using the established method.
基于24个正品药材样本一致的色谱特征，建立了虎杖的高效液相色谱 - 二极管阵列检测（HPLC-DAD）指纹图谱。分析了主要化学成分类型，即二苯乙烯类和蒽醌类，并将其纳入指纹图谱。通过HPLC-MS鉴定了虎杖的8个共有峰。所建立的指纹图谱用于区分虎杖与何首乌和大黄。尽管这三种药材都属于蓼科，但结果表明，使用所建立的方法可以将它们区分开来。
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