Liu Xiao-Qiu, Du Li-Li, Li Wei-Wei, Guan Hong-Feng, Liu Fang
Department of Analysis of Traditional Chinese Medicine, School of Traditional Chinese Medicine, Shenyang Pharmaceutical University, China.
Chem Pharm Bull (Tokyo). 2008 Jan;56(1):75-8. doi: 10.1248/cpb.56.75.
A qualitative and quantitative analysis method was established to improve quality assessment standards for Rhizoma Polygoni Bistortae (Polygonum bistorta L.) and differentiate commercial bistorta rhizome from closely related herbs by TLC and HPLC-DAD fingerprinting. Three compounds including phenolic acid and flavane were identified by comparison with standard compounds and quantified simultaneously by HPLC-DAD simultaneously. A comprehensive validation of the method that included sensitivity, linearity, repeatability and recovery was conducted. Paris polyphylla SM., a herb often mixed with Polygonum bistorta L. in China due to their same popular name "Caoheche" in history, was successfully distinguished by thin-layer chromatography (TLC) fingerprinting of the petroleum-soluble fraction. Polygonum paleaceum WALL., another herb often mixed with Polygonum bistorta L. due to their similar external appearances, was distinguished by HPLC fingerprinting.
建立了一种定性和定量分析方法，以提高拳参（Polygonum bistorta L.）的质量评估标准，并通过薄层色谱（TLC）和高效液相色谱-二极管阵列检测（HPLC-DAD）指纹图谱将市售拳参根茎与近缘草药区分开来。通过与标准化合物比较，鉴定出三种包括酚酸和黄烷的化合物，并同时通过HPLC-DAD进行定量。对该方法进行了包括灵敏度、线性、重复性和回收率在内的全面验证。七叶一枝花（Paris polyphylla SM.），在中国常因历史上有相同俗名“曹河车”而与拳参混淆，通过石油醚可溶部位的薄层色谱（TLC）指纹图谱成功区分。绵毛酸模叶蓼（Polygonum paleaceum WALL.），另一种常因外观相似而与拳参混淆的草药，通过HPLC指纹图谱进行区分。
