Ma Yun-tong, Wan De-guang, Song Liang-ke
Pharmacy School of Chengdu University of Traditional Chinese Medicine, Chengdu 610075, China.
Zhongguo Zhong Yao Za Zhi. 2006 Jun;31(12):972-4.
To establish the HPLC fingerprint of Rhizoma Polygoni Cuspidati (Polygonum cuspidatum).
The HPLC separation was carried with Diamonsil C18 column and eluted with a gradient from methanol and 0.1% phosphoric acid, the detection wavelength was at 230 nm and recording 70 min. The similarity of chromatograms was compared by mean of the software from Zhongnan University.
The constituents of P. cuspidatum were well separated by HPLC, and the similarity was above 0.80.
The method can be used for the study of fingerprints of Rhizoma Polygoni Cuspidati.
建立虎杖的高效液相色谱指纹图谱。
采用 Diamonsil C18 柱进行高效液相色谱分离，以甲醇和 0.1%磷酸为流动相进行梯度洗脱，检测波长为 230 nm，记录时间为 70 min。采用中南大学的软件对色谱图的相似度进行比较。
高效液相色谱法能很好地分离虎杖中的成分，相似度在 0.80 以上。
该方法可用于虎杖指纹图谱的研究。
