Perlas Leah A, Gibson Rosalind S
Food and Nutrition Research Institute, Department of Science and Technology, Bicutan, Taguig, Metro Manila, Philippines.
Matern Child Nutr. 2005 Oct;1(4):263-73. doi: 10.1111/j.1740-8709.2005.00037.x.
Philippine complementary foods are predominantly plant-based, with a low content of readily available iron, zinc, and calcium, and a relatively high amount of phytate, a potent inhibitor of mineral absorption. Some of the phytate is water soluble, and hence can be removed by soaking. In this study we have compared the iron, zinc, and calcium content, and estimated iron and zinc bioavailability of rice- and maize-based Filipino complementary foods prepared with and without soaking and/or enrichment with chicken liver, egg yolk, small soft-boned fish, and mung bean grits. Analysis of iron, zinc, and calcium were performed by atomic absorption spectrometry, and phytate (based on hexa-(IP6) and penta-inositol phosphate (IP5) by HPLC; corresponding [Phy]/[Fe] and [Phy]/[Zn] molar ratios were calculated as predictors of iron and zinc bioavailability. Addition of chicken liver, followed by egg yolk, resulted in the greatest increases in iron and zinc content for both the rice- and maize-based complementary foods, whereas addition of small dried fish with bones had the greatest effect on calcium. The IP5 + IP6 content and [Phy]/[Zn] molar ratios were higher in the maize- than rice-based complementary foods, and were reduced by soaking, although only the maize plus mung bean grits, with and without soaking, had [Phy]/[Zn] molar ratios above 15. Enrichment with animal protein or soaking has the potential to enhance the content of absorbable iron, zinc, and probably calcium to varying degrees in rice- and maize-based Philippine complementary foods.
菲律宾的辅食主要以植物为基础,其中易吸收的铁、锌和钙含量较低,而植酸盐含量相对较高,植酸盐是矿物质吸收的有效抑制剂。部分植酸盐可溶于水,因此可通过浸泡去除。在本研究中,我们比较了用鸡肝、蛋黄、小的带软骨头的鱼和绿豆粗粉进行浸泡和/或强化处理与未处理的菲律宾大米和玉米辅食中铁、锌和钙的含量,并估算了铁和锌的生物利用率。采用原子吸收光谱法对铁、锌和钙进行分析,通过高效液相色谱法测定植酸盐(基于六磷酸肌醇(IP6)和五磷酸肌醇(IP5));计算相应的[植酸盐]/[铁]和[植酸盐]/[锌]摩尔比作为铁和锌生物利用率的预测指标。添加鸡肝,其次是蛋黄,使大米和玉米辅食中的铁和锌含量增加最多,而添加带骨小鱼干对钙的影响最大。玉米辅食中的IP5 + IP6含量和[植酸盐]/[锌]摩尔比高于大米辅食,浸泡可降低其含量,不过只有玉米加绿豆粗粉,无论是否浸泡,其[植酸盐]/[锌]摩尔比均高于15。在菲律宾大米和玉米辅食中添加动物蛋白或进行浸泡有可能不同程度地提高可吸收铁、锌以及可能还有钙的含量。