Mohd Ridzuan M A R, Noor Rain A, Zhari I, Zakiah I
Herbal Medicine Research Center, Institute for Medical Research, Jalan Pahang,50588 Kuala Lumpur, Malaysia.
Trop Biomed. 2005 Dec;22(2):155-63.
In the present study we examined the effect of E. longifolia methanol extract (TA164) on the GSH levels of P. falciparum infected erythrocytes and uninfected erythrocytes. Our study on parasite growth shows the IC50 and IC75 values of TA164 to be 0.17 g/ml and 6 g/ml respectively while for BSO was 25.5 g/ml and 46.5 g/ml respectively. About 95% to 100% growth inhibition of P. falciparum infected erythrocyte was observed when treated with TA164 and BSO at 16 g/ml and 64 g/ml respectively. The study on GSH contents indicated that non-infected erythrocytes treated with 6 g/ml (IC75 values) of TA164 at 24 hours incubation showed less GSH content as compared to non-treated erythrocytes. A similar observation was seen on treated trophozoite infected erythrocyte (10% parasitemia) when treated with 6 g/ml at 3 hours incubation. Analysis of the GSH contents of parasite compartments treated with TA164 at the same concentration (6 g/ml) for 3 hours incubation indicated a reduction of GSH contents. At the same concentration, TA164 did not affect the GSH contents of enriched trophozoite infected erythrocytes (60-70% parasitemia). TA164 did affect the GSH content of non-infected erythrocyte at 24 hours (accept IC50 value) as well as the parasite compartments (trophozoite infected erythrocyte and parasite itself) but fails to affect the GSH content of enriched trophozoite infected erythrocyte.
在本研究中，我们检测了长叶红豆杉甲醇提取物（TA164）对恶性疟原虫感染红细胞和未感染红细胞中谷胱甘肽（GSH）水平的影响。我们对寄生虫生长的研究表明，TA164的IC50和IC75值分别为0.17 g/ml和6 g/ml，而丁硫氨酸亚砜胺（BSO）的IC50和IC75值分别为25.5 g/ml和46.5 g/ml。当分别用16 g/ml的TA164和64 g/ml的BSO处理时，观察到恶性疟原虫感染红细胞的生长抑制率约为95%至100%。对GSH含量的研究表明，在24小时孵育时用6 g/ml（IC75值）的TA164处理的未感染红细胞，与未处理的红细胞相比，GSH含量较低。当在3小时孵育时用6 g/ml处理受滋养体感染的红细胞（10%的寄生虫血症）时，也观察到了类似的现象。对用相同浓度（6 g/ml）的TA164处理3小时的寄生虫区室的GSH含量分析表明，GSH含量降低。在相同浓度下，TA164在24小时（接受IC50值）时不影响富含滋养体感染红细胞（60 - 70%寄生虫血症）的GSH含量，但确实影响未感染红细胞以及寄生虫区室（受滋养体感染的红细胞和寄生虫本身）的GSH含量。
