Serafin W E, Reynolds D S, Rogelj S, Lane W S, Conder G A, Johnson S S, Austen K F, Stevens R L
Department of Medicine, Harvard Medical School, Boston, Massachusetts.
J Biol Chem. 1990 Jan 5;265(1):423-9.
A novel 28,000 Mr serine protease, designated mouse mast cell protease-2 (MMCP-2), that is stored in the secretory granules of Kirsten sarcoma virus-immortalized mouse mast cells (KiSV-MC) has been identified and its NH2-terminal amino acid sequence has been determined. Analysis of a 953-base pair cDNA that encodes MMCP-2 revealed that this serine protease is a basically charged protein, possessing the histidine-aspartic acid-serine charge relay system that is characteristic of other serine proteases. DNA blot analysis using the full-length MMCP-2 cDNA indicated the existence of a family of highly related serine protease genes in the mouse genome. When the same DNA blot was probed with the 149-base pair KpnI----3' fragment of the cDNA, the probe hybridized to a single DNA fragment, thereby demonstrating that this 3' fragment could be used as a gene-specific probe. The presence of high levels of the MMCP-2 mRNA transcript in the intestines of nematode-infected mice, and its absence in mouse bone marrow-derived mast cells and peritoneal cavity-derived connective tissue mast cells, suggest that this member of the mouse mast cell protease family is preferentially expressed late in the differentiation of mucosal mast cells.
一种分子量为28000的新型丝氨酸蛋白酶，命名为小鼠肥大细胞蛋白酶-2（MMCP-2），已在 Kirsten 肉瘤病毒永生化小鼠肥大细胞（KiSV-MC）的分泌颗粒中被鉴定出来，并且其氨基末端氨基酸序列已被确定。对编码MMCP-2的953个碱基对的cDNA进行分析表明，这种丝氨酸蛋白酶是一种带碱性电荷的蛋白质，具有其他丝氨酸蛋白酶所特有的组氨酸-天冬氨酸-丝氨酸电荷中继系统。使用全长MMCP-2 cDNA进行DNA印迹分析表明，在小鼠基因组中存在一个高度相关的丝氨酸蛋白酶基因家族。当用该cDNA的149个碱基对的KpnI----3'片段对同一DNA印迹进行探测时，该探针与一个单一的DNA片段杂交，从而证明这个3'片段可以用作基因特异性探针。线虫感染小鼠的肠道中存在高水平的MMCP-2 mRNA转录本，而在小鼠骨髓来源的肥大细胞和腹腔来源的结缔组织肥大细胞中不存在，这表明小鼠肥大细胞蛋白酶家族的这个成员在黏膜肥大细胞分化后期优先表达。
