维生素D——人乳腺癌细胞系MCF-7中的代谢

Vitamin D--metabolism in the human breast cancer cell line MCF-7.

作者信息

Diesing Dagmar, Cordes Tim, Fischer Dorothea, Diedrich Klaus, Friedrich Michael

机构信息

University of Schleswig-Holstein, Department of Gynaecology and Obstetrics, 23627 Luebeck, Germany.

出版信息

Anticancer Res. 2006 Jul-Aug;26(4A):2755-9.

PMID:16886688

Abstract

BACKGROUND

The three main vitamin D metabolizing enzymes, vitamin D3-25-hydroxylase (25-OHase, 25-hydroxylase), 25-hydroxyvitamin D3-1alpha-hydroxylase (1alpha-OHase, 1alpha-hydroxylase) and 25-hydroxyvitamin D3-24-hydroxylase (24-OHase, 24-hydroxylase), have been described in malignant breast tissue. This in vitro study aimed to obtain more information regarding the regulation of these enzymes in the human breast cancer cell line MCF-7.

MATERIALS AND METHODS

Vitamin D receptor (VDR)- positive MCF-7 cells in culture were stimulated with the vitamin D metabolites vitamin D3, 25-hydroxyvitamin D3 and 1,25-dihydroxyvitamin D3 for 24, 48; 72 and 96 hours in physiological and supraphysiological concentrations. The expressions of 25-hydroxylase, 1alpha-hydroxylase and 24-hydroxylase and their changes after stimulation were assessed by real-time PCR.

RESULTS

The expression of 25-hydroxylase was slightly influenced by vitamin D3. The expression of 1alpha-hydroxylase was induced after stimulation with vitamin D3 and 25-hydroxyvitamin D3. Stimulation with 1,25-dihydroxyvitamin D3 markedly increased the expression of 24-hydroxylase time- and dose-dependently.

CONCLUSION

Our results demonstrated that MCF-7 cells are able to regulate the expression of 24-hydroxylase. This might be a mechanism for these tumor cells to protect themselves against the antiproliferative and apoptosis-inducing effects of calcitriol.

摘要

背景

三种主要的维生素D代谢酶，即维生素D3 - 25 - 羟化酶（25 - OHase，25 - 羟化酶）、25 - 羟基维生素D3 - 1α - 羟化酶（1α - OHase，1α - 羟化酶）和25 - 羟基维生素D3 - 24 - 羟化酶（24 - OHase，24 - 羟化酶），已在恶性乳腺组织中被描述。这项体外研究旨在获取更多关于这些酶在人乳腺癌细胞系MCF - 7中调节机制的信息。