维生素 D、钙二醇和钙三醇调节宫颈癌和卵巢癌细胞中的维生素 D 代谢酶。
Vitamin D, calcidiol and calcitriol regulate vitamin D metabolizing enzymes in cervical and ovarian cancer cells.
机构信息
Department of Obstetrics and Gynecology, University of Lübeck, D-23538 Lübeck, Germany.
出版信息
Anticancer Res. 2010 Nov;30(11):4429-34.
BACKGROUND
The vitamin D metabolizing enzymes 25-, 1α- and 24-hydroxylase are expressed in malignant cells of the cervix and the ovaries. The aim of this study was to obtain further information about the regulation of the aforementioned enzymes by vitamin D, calcidiol and calcitriol in cervical and ovarian cancer.
MATERIALS AND METHODS
The human cervical adenocarcinoma cell line HeLa and the human ovarian adenocarcinoma cell line OVCAR-3 were incubated with vitamin D, calcidiol and calcitriol. The influence of vitamin D and its metabolites on the expression of 25-, 1α- and 24-hydroxylase was assessed by real-time RT-PCR.
RESULTS
Calcitriol significantly increased the 24-hydroxylase mRNA levels in HeLa and OVCAR-3 cells. The expression of 25- and 1α-hydroxylase was not regulated in a statistically significant manner.
CONCLUSION
These results suggest that in HeLa as well as OVCAR-3 cell lines, the metabolism of vitamin D is regulated via the expression of the catabolizing 24-hydroxylase.
背景
维生素 D 代谢酶 25-羟化酶、1α-羟化酶和 24-羟化酶在宫颈和卵巢的恶性细胞中表达。本研究旨在进一步了解维生素 D、骨化二醇和骨化三醇对宫颈和卵巢癌细胞中上述酶的调控。
材料与方法
用人宫颈腺癌细胞系 HeLa 和人卵巢腺癌细胞系 OVCAR-3 进行孵育，用维生素 D、骨化二醇和骨化三醇进行孵育。通过实时 RT-PCR 评估维生素 D 及其代谢物对 25-、1α-和 24-羟化酶表达的影响。
结果
骨化三醇显著增加了 HeLa 和 OVCAR-3 细胞中 24-羟化酶 mRNA 水平。25-羟化酶和 1α-羟化酶的表达未受到统计学显著调控。
结论
这些结果表明，在 HeLa 以及 OVCAR-3 细胞系中，维生素 D 的代谢通过代谢酶 24-羟化酶的表达来调节。
Zotero 插件