Mateos E, Valle J, Píriz S, Cerrato R, Jiménez R, Perea Remujo A, Vadillo S
Departamento de Medicina y Sanidad Animal, Microbiología e Inmunología, Facultad de Veterinaria, Universidad de Extremadura, Cáceres, Spain.
Anaerobe. 1998 Oct;4(5):213-8. doi: 10.1006/anae.1998.0166.
A microbiological study was made of 100 strains of Fusobacterium necrophorum isolated from hepatic abscesses in bovine and ovine herds. Differences between the biological activity and ribotypes within the two F. necrophorum subspecies were studied. Conventional methods identified 89 isolates as F. necrophorum subsp. necrophorum and 11 as F. necrophorum subsp. funduliforme. For ribotyping, 50 strains (35 F.n. subsp. necrophorum, 11 F.n. subsp. funduliforme and 4 reference strains) were digested with restriction endonucleases (HindIII, EcoRI and BamHI) and examined after hybridization with digoxigenin-labelled cDNA probe transcribed from a 16 and 23S rRNAs from Escherichia coli. The most discriminating restriction endonuclease enzymes for ribotyping were EcoRI and BamHI. The presence or absence of two distinct band of 5 kb (EcoRI) and 10.5 kb (BamHI) differentiated the two subspecies. This technique also revealed genetic differences between isolates which could be used in the epidemiological study of clinical processes caused by F. necrophorum.
对从牛群和羊群肝脓肿中分离出的100株坏死梭杆菌进行了微生物学研究。研究了两个坏死梭杆菌亚种在生物活性和核糖型之间的差异。传统方法鉴定出89株分离株为坏死梭杆菌坏死亚种，11株为坏死梭杆菌底形亚种。对于核糖分型，用限制性内切酶（HindIII、EcoRI和BamHI）消化50株菌株（35株坏死梭杆菌坏死亚种、11株坏死梭杆菌底形亚种和4株参考菌株），并用从大肠杆菌16S和23S rRNA转录的地高辛标记cDNA探针杂交后进行检测。用于核糖分型的最具鉴别力的限制性内切酶是EcoRI和BamHI。5 kb（EcoRI）和10.5 kb（BamHI）两条不同条带的有无区分了两个亚种。该技术还揭示了分离株之间的遗传差异，可用于坏死梭杆菌引起的临床过程的流行病学研究。
