Shinjo T, Misawa N, Goto Y
Department of Veterinary Microbiology, Miyazaki University, Japan.
APMIS. 1996 Jan;104(1):75-8.
Haemolytic activity of two subspecies of Fusobacterium necrophorum was compared in vitro and in vivo. F. necrophorum subsp. necrophorum (Fnn) showed a stronger activity than F. necrophorum subsp. funduliforme (Fnf) in vitro. Haemolytic activity of Fnn and Fnf was 57.97%+/-1.90 and 17.33%+/-1.44, respectively, compared to complete haemolysis by distilled water. In the mice injected with Fnn, haemolysin was detected in the liver at a titre of from 1 : 16 to 1 : 128, and Fnn was recovered from all mice at a viable bacterial count of 10(5) to 10(6) cells per gram liver tissue. In the mice injected with Fnf, haemolysin titre was <1 : 2 to 1 : 32. No liver abscess was formed. The viable count of recovered bacteria was 10(3) to 10(5) cells per gram, except for two mice in which no Fnf was detected. The results suggest that haemolysin might be a virulence factor in this species.
对坏死梭杆菌两个亚种的溶血活性进行了体外和体内比较。坏死梭杆菌坏死亚种（Fnn）在体外显示出比坏死梭杆菌底形亚种（Fnf）更强的活性。与蒸馏水完全溶血相比，Fnn和Fnf的溶血活性分别为57.97%±1.90和17.33%±1.44。在注射Fnn的小鼠中，在肝脏中检测到溶血素，效价为1:16至1:128，并且从所有小鼠中回收的Fnn的活菌数为每克肝脏组织10(5)至10(6)个细胞。在注射Fnf的小鼠中，溶血素效价<1:2至1:32。未形成肝脓肿。回收细菌的活菌数为每克10(3)至10(5)个细胞，除了两只未检测到Fnf的小鼠。结果表明溶血素可能是该物种的一种毒力因子。
