Zhainazarov A B, Kolesnikov S S
Institute of Biological Physics, USSR Academy of Sciences, Pushchino, Moscow Region.
FEBS Lett. 1990 Jan 15;260(1):149-51. doi: 10.1016/0014-5793(90)80089-2.
Integral cGMP-dependent currents as well as activity of single cGMP-activated channels in plasma membrane of rod outer segment (ROS) were recorded using the patch-clamp method. The dependence of integral currents on cGMP concentration is shown to be bell-shaped. Decrease in cGMP-dependent conductance at high cGMP concentration results from a decrease in channel opening probability. Thus, cGMP in high concentrations inactivates cGMP-dependent conductance.
使用膜片钳方法记录了视杆外段（ROS）质膜中完整的环鸟苷酸（cGMP）依赖性电流以及单个cGMP激活通道的活性。完整电流对cGMP浓度的依赖性呈钟形。高cGMP浓度下cGMP依赖性电导的降低是由于通道开放概率的降低。因此，高浓度的cGMP会使cGMP依赖性电导失活。
