Bina Minou
Department of Chemistry, Purdue University, West Lafayette, IN, USA.
Methods Mol Biol. 2006;338:21-9. doi: 10.1385/1-59745-097-9:21.
The completion of whole genome sequencing projects offers the opportunity to examine the organization of genes and the discovery of evolutionarily related genes in a given species. For the beginners in the field, through a specific example, this chapter provides a step-by-step procedure for identifying paralogous genes, using the genome browser at UCSC (http://genome.ucsc.edu/). The example describes identification and mapping in the human genome, the paralogs of TCF12/HTF4. The example identifies TCF3 and TCF4 as paralogs of the TCF12/HTF4 gene. The example also identifies a related sequence, corresponding to a pseudogene, in one of the introns of the JAK2 gene. The procedure described should be applicable to the discovery and creation of maps of paralogous genes in the genomic DNA sequences that are available at the genome browser at UCSC.
全基因组测序项目的完成提供了一个机会，可用于研究特定物种中基因的组织方式以及发现进化相关基因。对于该领域的初学者，本章通过一个具体示例，提供了使用加州大学圣克鲁兹分校（UCSC）的基因组浏览器（http://genome.ucsc.edu/）来识别旁系同源基因的逐步流程。该示例描述了在人类基因组中对TCF12/HTF4旁系同源基因的识别和定位。该示例确定TCF3和TCF4是TCF12/HTF4基因的旁系同源基因。该示例还在JAK2基因的一个内含子中鉴定出了一个与假基因相对应的相关序列。所描述的流程应适用于在UCSC基因组浏览器上可获取的基因组DNA序列中发现和绘制旁系同源基因图谱。
