Bhagwat Medha, Young Lynn, Robison Rex R
National Institutes of Health Library, National Institutes of Health, Bethesda, Maryland.
Curr Protoc Bioinformatics. 2012 Mar;Chapter 10:10.8.1-10.8.24. doi: 10.1002/0471250953.bi1008s37.
The BLAST-Like Alignment Tool (BLAT) is used to find genomic sequences that match a protein or DNA sequence submitted by the user. BLAT is typically used for searching similar sequences within the same or closely related species. It was developed to align millions of expressed sequence tags and mouse whole-genome random reads to the human genome at a higher speed. It is freely available either on the Web or as a downloadable stand-alone program. BLAT search results provide a link for visualization in the University of California, Santa Cruz (UCSC) Genome Browser, where associated biological information may be obtained. Three example protocols are given: using an mRNA sequence to identify the exon-intron locations and associated gene in the genomic sequence of the same species, using a protein sequence to identify the coding regions in a genomic sequence and to search for gene family members in the same species, and using a protein sequence to find homologs in another species.
类BLAST比对工具（BLAT）用于查找与用户提交的蛋白质或DNA序列相匹配的基因组序列。BLAT通常用于在同一物种或亲缘关系密切的物种中搜索相似序列。它的开发目的是将数百万个表达序列标签和小鼠全基因组随机读数以更高的速度与人类基因组进行比对。它既可以在网上免费获取，也可以作为可下载的独立程序获得。BLAT搜索结果提供了一个链接，用于在加利福尼亚大学圣克鲁兹分校（UCSC）基因组浏览器中进行可视化，在那里可以获取相关的生物学信息。给出了三个示例方案：使用mRNA序列在同一物种的基因组序列中识别外显子-内含子位置和相关基因，使用蛋白质序列在基因组序列中识别编码区域并在同一物种中搜索基因家族成员，以及使用蛋白质序列在另一个物种中查找同源物。
