Marcotte G V, Millard P J, Fewtrell C
Department of Pharmacology, New York State College of Veterinary Medicine, Cornell University, Ithaca 14853.
J Cell Physiol. 1990 Jan;142(1):78-88. doi: 10.1002/jcp.1041420111.
Release of calcium from intracellular stores of rat basophilic leukemia cells was monitored using the fluorescent probe chlortetracycline. The ability of chlortetracycline to indicate release from intracellular calcium stores was initially validated. The decrease of chlortetracycline fluorescence upon antigen-stimulation was not the result of secretion of granule-associated dye or of changes in the properties of the membranes. The chlortetracycline fluorescence signal was not influenced by Ca2+ influx across the plasma membrane. Results obtained from these chlortetracycline fluorescence measurements corresponded well with 45Ca efflux data, an indirect measurement of release of calcium from stores. Chlortetracycline was used to examine the rate of antigen-induced release of calcium from stores, the depletion of intracellular calcium stores by EGTA, and the relationship between the antigen-stimulated release of stored calcium and exocytosis. Chlortetracycline was shown to be a useful qualitative indicator for the release of intracellular calcium with a relatively rapid response time.
使用荧光探针金霉素监测大鼠嗜碱性白血病细胞胞内钙库中钙的释放。金霉素指示胞内钙库释放钙的能力首先得到验证。抗原刺激后金霉素荧光的降低并非颗粒相关染料分泌或膜性质改变的结果。金霉素荧光信号不受跨质膜Ca2+内流的影响。这些金霉素荧光测量结果与45Ca外流数据（从钙库释放钙的间接测量）非常吻合。金霉素用于检测抗原诱导的钙从钙库释放的速率、EGTA对胞内钙库的耗竭以及储存钙的抗原刺激释放与胞吐作用之间的关系。金霉素被证明是一种有用的定性指标，用于检测胞内钙的释放，响应时间相对较快。
