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有丝分裂原刺激的人外周血淋巴细胞中细胞内钙离子的重新分布

Redistribution of intracellular Ca2+ in mitogen-stimulated human peripheral blood lymphocytes.

作者信息

Gukovskaya A S, Zinchenko V P, Ryabichenko V V, Astashkin E I

出版信息

Gen Physiol Biophys. 1983 Oct;2(5):395-407.

PMID:6432630
Abstract

The fluorescent probe chlortetracycline (CTC) was used to investigate redistribution of intracellular Ca2+ in concanavalin A (Con A)-stimulated human peripheral blood lymphocytes. The addition of the mitogen to CTC-equilibrated lymphocytes induced (within 10 to 15 minutes) a Con A-concentration dependent decrease in CTC fluorescence indicating the release of membrane-bound Ca2+. The effect was independent of the level of extracellular Ca2+ and could be observed in the presence of EGTA; it was suppressed by the metabolic inhibitors FCCP, antimycin and sodium cyanide. Analysis of the excitation spectra of CTC fluorescence indicated that the observed effect is caused by redistribution of intracellular Ca2+ rather than Mg2+. Thus the lectin interaction with the lymphocyte plasma membrane results in Ca2+ release into the cytosol from the intracellular stores.

摘要

荧光探针金霉素(CTC)用于研究伴刀豆球蛋白A(Con A)刺激的人外周血淋巴细胞内Ca2+的重新分布。向用CTC平衡的淋巴细胞中添加促有丝分裂原会在10至15分钟内诱导出Con A浓度依赖性的CTC荧光降低,这表明膜结合Ca2+的释放。该效应与细胞外Ca2+水平无关,并且在EGTA存在的情况下也可观察到;它被代谢抑制剂羰基氰化物间氯苯腙(FCCP)、抗霉素和氰化钠抑制。对CTC荧光激发光谱的分析表明,观察到的效应是由细胞内Ca2+而非Mg2+的重新分布引起的。因此,凝集素与淋巴细胞质膜的相互作用导致Ca2+从细胞内储存库释放到细胞质中。

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