Cammer W
Department of Neurology, Albert Einstein College of Medicine, Bronx, NY 10461.
J Neuroimmunol. 1990 Feb;26(2):173-8. doi: 10.1016/0165-5728(90)90088-5.
Glutamine synthetase (GS) immunoreactivity is frequently used as an astroglial 'marker'. However, when sections of adult rat spinal cords were immunostained with antibodies against sheep glutamine synthetase, intense immunofluorescence was observed in cells resembling oligodendrocytes. In white matter in the rat brain GS immunostaining was also found in ovoid oligodendrocyte-like cells, whereas in gray matter in the same tissue sections GS immunostaining was found in astrocytes. Like the antibodies against sheep GS, antibodies against rat GS also immunostained putative oligodendrocytes, and colocalization with 2',3'-cyclic nucleotide-3'-phosphohydrolase in spinal cord supported the designation of the GS-positive cells as oligodendrocytes.
谷氨酰胺合成酶（GS）免疫反应性常被用作星形胶质细胞的“标志物”。然而，当用抗绵羊谷氨酰胺合成酶抗体对成年大鼠脊髓切片进行免疫染色时，在类似少突胶质细胞的细胞中观察到强烈的免疫荧光。在大鼠脑白质中，也在卵形的少突胶质细胞样细胞中发现了GS免疫染色，而在同一组织切片的灰质中，GS免疫染色则出现在星形胶质细胞中。与抗绵羊GS抗体一样，抗大鼠GS抗体也对假定的少突胶质细胞进行了免疫染色，并且在脊髓中与2',3'-环核苷酸-3'-磷酸水解酶的共定位支持将GS阳性细胞认定为少突胶质细胞。
