Norenberg M D
J Histochem Cytochem. 1979 Mar;27(3):756-62. doi: 10.1177/27.3.39099.
The results of a light microscopic immunohistochemical study of glutamine synthetase in rat nervous system are presented. In all sites studied the enzyme was confined to astrocytes. Except for trace amounts in ependymal cells, the enzyme was not observed in other cells of the nervous system including neurons, choroid plexus, third ventricular tanycytes, subependymal cells and mesodermally-derived elements. The intensity of astrocyte staining varied in different regions with the greatest degree noted in the hippocampus and cerebellar cortex while the least was noted in brain stem, deep cerebellar nuclei and spinal cord. The glutamine synthetase content correlated well with sites of suspected glutamergic activity in keeping with the view of a critical role of astrocytes in the regulation of the putative neurotransmitter glutamic acid.
本文展示了大鼠神经系统中谷氨酰胺合成酶的光镜免疫组织化学研究结果。在所研究的所有部位，该酶均局限于星形胶质细胞。除室管膜细胞中有微量存在外，在包括神经元、脉络丛、第三脑室室管膜细胞、室管膜下细胞和中胚层来源成分在内的神经系统其他细胞中均未观察到该酶。星形胶质细胞染色强度在不同区域有所不同，在海马体和小脑皮质中最为明显，而在脑干、小脑深部核团和脊髓中则最不明显。谷氨酰胺合成酶含量与疑似谷氨酸能活动部位密切相关，这与星形胶质细胞在调节假定神经递质谷氨酸中起关键作用的观点一致。
