Nowakowska Dorota, Gołab Elzbieta, Czichos Ewa, Krekora Michał, Wilczyński Jan
Klinika Medycyny Matczyno-Płodowej, ICZMP, ul. Rzgowska 281/289, 93-338 Lódź.
Wiad Parazytol. 2002;48(3):301-9.
Since isolation of Toxoplasma gondii from human placenta strongly correlates with fetal infection, the aims of the study were: to detect fragments of T. gondii B1 gene in human placentae by PCR and to evaluate their pathology. 36 placentae included in three groups were obtained: group I (n = 7) from pregnancies with prenatal diagnosis of fetal toxoplasmosis; II (n = 17) from women with serologic features of primary infection during pregnancy; III (n and 13) from pregnancies with fetal T. gondii infection based on clinical signs. T. gondii DNA was found in 2/4 samples from the I group and in 1/14 from the II group. Villitis was identified in 3/15 other placentae from the II group. In the III group we did not recognize neither T. gondii DNA nor villitis. We consider PCR and pathologic evaluations of placentae as the two complementary methods. PCR can be especially helpful in pregnancies not screened against T. gondii as positive result in placenta can confirm mother's primary infection.
由于从人胎盘中分离出刚地弓形虫与胎儿感染密切相关，本研究的目的是：通过聚合酶链反应（PCR）检测人胎盘中刚地弓形虫B1基因片段，并评估其病理学特征。收集了纳入三组的36份胎盘：第一组（n = 7）来自产前诊断为胎儿弓形虫病的孕妇；第二组（n = 17）来自孕期有原发性感染血清学特征的女性；第三组（n = 13）来自根据临床症状诊断为胎儿刚地弓形虫感染的孕妇。在第一组的2/4份样本和第二组的1/14份样本中发现了刚地弓形虫DNA。在第二组的其他15份胎盘中，有3份发现绒毛炎。在第三组中，未检测到刚地弓形虫DNA，也未发现绒毛炎。我们认为胎盘的PCR检测和病理学评估是两种互补的方法。PCR检测对未进行弓形虫筛查的孕妇特别有用，因为胎盘中的阳性结果可以确认母亲的原发性感染。
