Czuba B, Pendzich J, Gola J, Mazurek U, Sławska H, Kamiński K, Wilczok T
Katedra i Klinika Patologii Ciazy i Rozrodczości w Zabrzu, Slaskiej Akademii Medycznej, 41-800 Zabrze, ul. Janika 18.
Wiad Parazytol. 2001;47 Suppl 1:91-7.
Routine serological diagnosis of toxoplasmosis provides high sensitivity, but not high specificity. The high sensivity combined with high specifity offered by PCR-TaqMann as well as the degree of infection led us to investigate the presence and levels of T. gondii DNA in amniotic fluid, maternal and neonatal blood in cases of pregnancy where infection with this agent was suspected. Samples of amniotic fluid and blood were taken from pregnant women. Postnatal blood samples were also taken from their infants. Presence and levels of toxoplasma DNA was investigated using PCR-TaqMann. PCR products were detected by electrophoresis on polyacrylamide gel. The PCR-TaqMann test is highly sensitive, specific and useful method allowing detection of the parasite genome and assessement of its level.
弓形虫病的常规血清学诊断具有较高的敏感性，但特异性不高。PCR-TaqMann检测所具有的高敏感性与高特异性以及感染程度，促使我们对疑似感染该病原体的孕妇病例的羊水、母血和新生儿血液中弓形虫DNA的存在情况及水平进行调查。从孕妇身上采集羊水和血液样本。还从她们的婴儿身上采集产后血样。使用PCR-TaqMann检测法调查弓形虫DNA的存在情况及水平。通过聚丙烯酰胺凝胶电泳检测PCR产物。PCR-TaqMann检测法是一种高度敏感、特异且有用的方法，可用于检测寄生虫基因组并评估其水平。
