Skotarczak Bogumiła, Sawczuk Marek
Katedra Genetyki, Wydział Nauk Przyrodniczych, Uniwersytet Szczeciński, Al. Piastów 40B, 71-065 Szczecin.
Wiad Parazytol. 2003;49(3):273-80.
The aim of present study was to evaluate acquisition risk of babesiosis in human population exposed to ticks Ixodes ricinus by examination of Babesia microti DNA occurrence in ticks of all development stages. The polymerase chain reaction (PCR) was applied to estimate the occurrence of DNA Babesia microti in Ixodes ricinus. The Bab1 and Bab4 primers were used to amplify fragment. 238bp in length, of 18S rRNA gene for small ribosomal subunit. Amplicons were electroforeticaly separated in agarose gels. Ticks were collected in year 1999 and 2000, twice in each year in spring-summer (May-July) and summer-autumn (August-October) seasons from Goleni6w Forest and Pobierowo. These places have been classified as people attendance and tourist areas. The 716 I. ricinus ticks were collected in 1999 with 61.3% of nymphs, 17.8% larvae, 10.9% females and 9.9% males. Highest range of infection was observed in females--28.8% studied, than males--18.3%, nymphs--7.7% and larvae--3.l1%. The total number of 416 I. ricinus was collected in year 2000 with 64% of nymphs. 13.4%/males. 11.9% females and 10.7% larvae. The infection with Babesia microtri occurred only in three nymphs, which was 0.7% of studied population.
本研究的目的是通过检测蓖麻硬蜱各个发育阶段中微小巴贝斯虫DNA的存在情况，评估暴露于蓖麻硬蜱的人群感染巴贝斯虫病的风险。应用聚合酶链反应（PCR）来估计微小巴贝斯虫DNA在蓖麻硬蜱中的存在情况。使用Bab1和Bab4引物扩增长度为238bp的小核糖体亚基18S rRNA基因片段。扩增产物在琼脂糖凝胶中进行电泳分离。蜱虫于1999年和2000年采集，每年在春夏（5月至7月）和夏秋（8月至10月）季节从戈莱尼奥夫森林和波别罗沃采集两次。这些地方已被归类为有人活动和旅游区域。1999年共采集了716只蓖麻硬蜱，其中若虫占61.3%，幼虫占17.8%，雌蜱占10.9%，雄蜱占9.9%。观察到雌蜱的感染率最高——在所研究的雌蜱中为28.8%，其次是雄蜱——18.3%，若虫——7.7%，幼虫——3.1%。2000年共采集了416只蓖麻硬蜱，其中若虫占64%，雄蜱占13.4%，雌蜱占11.9%，幼虫占10.7%。微小巴贝斯虫感染仅发生在三只若虫中，占所研究种群的0.7%。
