Wójcik-Fatla Angelina, Cisak Ewa, Chmielewska-Badora Jolanta, Zwoliński Jacek, Buczek Alicja, Dutkiewicz Jacek
Department of Occupational Biohazards, Institute of Agricultural Medicine, Jaczewskiego 2, 20-090 Lublin, Poland.
Ann Agric Environ Med. 2006;13(2):319-22.
A total of 1,367 Ixodes ricinus ticks collected from 5 districts of the Lublin region (eastern Poland) were examined for the presence of Babesia microti DNA by PCR and nested-PCR. As many as 74 ticks (5.4 %) were found to be infected with Babesia microti. The infection rate varied significantly with stage/sex of ticks (chi (2) =16.48543, df=2, p < 0.000264). The infection rates in females and males amounted to 6.4 % and 8.8 % respectively and were significantly higher (p=0.006 and p=0.0001 respectively) compared to minimum infection rate in nymphs that was equal to 2.8 %. The prevalence of infection showed also a significant variability depending on geographic location within the Lublin region (chi(2) =18.62812, df=4, p < 0.000932). The highest rates of infection with Babesia microti were noted in ticks collected from the areas of Puławy district situated in the northern part of region and the suburban Lublin district situated in the central part of the region (8.0 % and 7.3 % respectively). Mediocre infection rates (respectively 3.4 % and 3.3 %) were found in ticks from the Parczew and Włodawa districts situated in eastern part of the region and covered with humid lakeland forests. The lowest infection rate (0.5 %) was noted in ticks from the Zamość district situated in southern part of the region. In conclusion, the infection rate of Ixodes ricinus ticks with Babesia microti found in this study is higher compared to the majority of data reported by Polish and other European authors, and indicates a potential risk of human infection during occupational or recreational exposure to tick bite.
对从波兰东部卢布林地区5个区采集的共计1367只蓖麻硬蜱进行了PCR和巢式PCR检测，以确定是否存在微小巴贝斯虫DNA。多达74只蜱（5.4%）被发现感染了微小巴贝斯虫。感染率随蜱的阶段/性别有显著差异（χ²=16.48543，自由度=2，p<0.000264）。雌性和雄性的感染率分别为6.4%和8.8%，与若虫的最低感染率（2.8%）相比显著更高（分别为p=0.006和p=0.0001）。感染率还因卢布林地区内的地理位置而有显著差异（χ²=18.62812，自由度=4，p<0.000932）。在从该地区北部的普瓦维区和中部的卢布林郊区采集的蜱中，微小巴贝斯虫感染率最高（分别为8.0%和7.3%）。在该地区东部、覆盖着湿润湖泊森林的帕尔采夫区和沃乌达瓦区的蜱中，感染率中等（分别为3.4%和3.3%）。在该地区南部的扎莫希奇区的蜱中，感染率最低（0.5%）。总之，本研究中发现的蓖麻硬蜱感染微小巴贝斯虫的比率高于波兰和其他欧洲作者报告的大多数数据，表明在职业或娱乐活动中接触蜱叮咬时存在人类感染的潜在风险。
